DNA Sequence Tools Protein Sequence Tools Lab Tools Data Manipulation Tools Biomechanic-->DNA Tools-->Oligo Calc

Hi, If you give me an oligonucleotide sequence (ssDNA, frequently used for PCR), I will tell you: the length and molcular weight %gc, ATGC counts, the extiction coefficient at 260nm and the melting temperature. If you have taken a reading at 260nm (dont forget the dilution factor!), I will also be able to tell you your oligo concentration and how to dilute it to get a specific concentration. Enter sequence below... Or select a file: A260: Dilution Factor: ug/mL(ssDNA): 0.00 pmol/uL(ssDNA): 0.00 Length: 0 %GC: 0 A's: 0 T's: 0 G's: 0 C's: 0 Mol. Wt.(g/M): 0.0 Melt. Temp.(oC): 0.0 Add 0.00uL to uL to get pmol/uL Molar Extinction Coefficient: 0.00 1 OD is: 0picoMolar